Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The axonal shafts of neurons contain bundled microtubules, whereas extending growth cones contain unbundled microtubule filaments, suggesting that localized activation of microtubule-associated proteins (MAP) at the transition zone may bundle these filaments during axonal growth. Dephosphorylation is thought to lead to MAP activation, but specific molecular pathways have remained elusive. We find that Spinophilin, a Protein-phosphatase 1 (PP1) targeting protein, is responsible for the dephosphorylation of the MAP Doublecortin (Dcx) Ser 297 selectively at the "wrist" of growing axons, leading to activation. Loss of activity at the "wrist" is evident as an impaired microtubule cytoskeleton along the shaft. These findings suggest that spatially restricted adaptor-specific MAP reactivation through dephosphorylation is important in organization of the neuronal cytoskeleton.

Original publication

DOI

10.1016/j.cell.2007.03.023

Type

Journal article

Journal

Cell

Publication Date

04/05/2007

Volume

129

Pages

579 - 591

Keywords

Actins, Animals, Axons, Cells, Cultured, Corpus Callosum, Cyclin-Dependent Kinase 5, Hippocampus, Humans, Magnetic Resonance Imaging, Male, Mice, Mice, Inbred Strains, Mice, Knockout, Microfilament Proteins, Microtubule-Associated Proteins, Microtubules, Nerve Tissue Proteins, Neurites, Neurons, Neuropeptides, Phosphoprotein Phosphatases, Phosphorylation, Serine