Search results
Found 12119 matches for
A microfabricated device for sizing and sorting DNA molecules.
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.
Assessing the visual appeal of real/AI-generated food images
A study designed to investigate the ability of individuals to differentiate between AI-generated and authentic food images, as well as the impact of disclosing this information on the consumer perception of the appeal of these images is reported. Two online experiments were conducted with real and AI-generated food images stretching across the unprocessed, processed, and ultra-processed food continuum. Study 1 was designed to assess the accuracy with which people could identify AI-generated food images while Study 2 explored how the disclosure of an image's origin influenced the appeal of the depicted food. The participants in Study 1 found it very easy to recognize the AI-generated images, particularly in the case of ultra-processed foods. Notably, without disclosure, the AI-generated images were often preferred. At the same time, however, disclosing that a food image was genuine significantly boosted its appeal, whereas the revelation that it had been generated by AI mitigated this effect. These insights help to understand consumer psychology in the rapidly-evolving digital food marketing landscape, highlighting the nuanced effects of technological advancements in AI image-generation on human perception.
Individual differences in sensitivity to taste-shape crossmodal correspondences
People generally associate curved and symmetrical shapes with sweetness, while associating angular and asymmetrical shapes with the other basic tastes (e.g., sour, bitter). However, these group-level taste-shape correspondences likely conceal important variation at an individual-level. We examined the extent to which individuals vary in their sensitivity to crossmodal correspondence between curvature and symmetry, on the one hand, and the five basic taste qualities (sweet, bitter, salty, sour, and umami), on the other. In Experiment 1, participants matched shapes (curved vs. angular, symmetrical vs. asymmetrical) and taste words. In Experiment 2, participants performed a similar task, though this time using actual tastants. Given that people differ in their hedonic experience of such shapes and tastes, we also measured participants’ liking for each taste and shape separately. The results replicate the general crossmodal correspondences between curved-sweet and symmetrical-sweet stimuli. Furthermore, participants tended to match sour and bitter tastes with angular and asymmetrical stimuli. However, these group-level taste-shape correspondences coexist alongside substantial variation at the level of the individual. While some participants consistently matched specific tastes with curved and symmetrical stimuli, others consistently matched these tastes with angular and asymmetrical stimuli, or else did not show these taste-shape correspondences. Liking for curved and symmetrical stimuli was higher than for angular and asymmetrical stimuli. However, participants also differed considerably in the extent to which these visual features affected their liking. Overall, our findings highlight the substantial individual differences that are associated with the degree to which people associate and like shapes and tastes.
An integrated microfabricated cell sorter.
We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is approximately 100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.
The imitation game–exploring the double-grip analysis for creating analog wines
In this pilot study, we investigated the use of the Double-Grip Analysis method as a tool to create analogue wines. The study involved four groups of sommeliers using the method to identify critical analytical and analogical attributes in four commercial wines. The basis of communication with this method is via the use of analogies, metaphors, and practical examples that emerge during the analysis and the dialogue. An overmatured rosé wine with mature flavours and low acidity served as the base for all groups when replicating the commercial counterparts making analogue wines. At first, the study aimed to assess the sommeliers’ ability to use this method in blending and wine production. In addition, the study explored the acceptability of these analogue wines, as they aim to replicate sensory characteristics of commercial counterparts, providing environmentally friendly alternatives. A panel of 20 consumers evaluated the quality of the wines as guests at a blind tasting dinner. The results help to shed light on the effectiveness of the Double-Grip Analysis Method, while at the same time providing insights into the acceptability of these analogue products amongst consumers. This research contributes to the field of culinary exploration and sustainable alternatives in the wine industry.
How Expression and Context Determine Second-person Judgments of Emotion
Within the field of Affective Computing, facial expressions have traditionally been used as a means of inferring valence and emotions. Most studies have focused on interpreting facial expressions as an isolated signal, typically training algorithms with annotators with a 3rd-person point of view, often without access to the original context. When the context is provided, recent research highlights that the interpretation annotators assign to facial expressions is sometimes more strongly influenced by the context than the facial expression. But even when the context is provided, annotators are psychologically and physically distant from the original setting that produced the emotion. In this paper, we explore how context and facial expressions shape 2nd-person interpretation of facial expressions and compare this to 1st-person self-report. Results show that both expression and context contribute to self and other impressions but in different ways. Expressions and context are independent predictors of 1st-person judgments but interact to determine 2nd. person judgments. In particular, the way players interpret their partner's facial cues changes dramatically based on what just occurred in the game. We discuss the implication of these findings for automatic emotion recognition methods.
School‐based anti‐bullying approaches for children and young people with special educational needs and disabilities: A systematic review and synthesis
AbstractMainstream anti‐bullying interventions can reduce primary school‐level victimisation by 15–16% and bullying perpetration by 19%–20% (Aggression and Violent Behavior, 2019; 45: 111–133). Less is known about anti‐bullying interventions for pupils with special educational needs and disabilities (SEND) even though they are at least 2–4 times more likely to be involved in bullying. This systematic review aimed to identify reported anti‐bullying approaches for pupils with SEND, what the evidence is for these approaches reducing bullying and which design factors are linked to a reduction in bullying. We searched 10 databases and four grey literature sources for articles that evaluated school‐based anti‐bullying strategies for children and young people aged 4–18 years with SEND. This review included 15 studies and used the Mixed Methods Appraisal Tool for quality and risk of bias. Ten reported a reduction in bullying involvement, but the evidence was variable. A further 27 articles formed a ‘suggested strategies’ review which synthesised articles without evaluations of interventions but that suggested anti‐bullying strategies for use with pupils with SEND. The main suggestion was encouraging social skills and networks. Interventions should be evaluated for feasibility, acceptability and effectiveness. High‐quality randomised controlled trials are required to build an evidence base to support pupils with SEND.
Solutions Trial: Solution Focused Brief Therapy (SFBT) in 10–17-year-olds presenting at police custody: a randomised controlled trial
Abstract Background Within England, children and young people (CYP) who come into police custody are referred to Liaison and Diversion (L&D) teams. L&D teams have responsibility for liaising with healthcare and other support services while working to divert CYP away from the criminal justice system but have traditionally not provided targeted psychological interventions to CYP. Considering evidence that Solution Focused Brief Therapy (SFBT) leads to a reduction in internalising and externalising behaviour problems in CYP, the aim of this randomised controlled trial (RCT) was to determine whether there is a difference between services as usual (SAU) plus SFBT offered by trained therapists working within a L&D team, and SAU alone, in reducing offending behaviours in 10–17-year-olds presenting at police custody. Methods Design: two-arm individually RCT with internal pilot and process evaluation. Participants: N = approximately 448 CYP aged 10–17 years presenting at one of three police custody suites in the area served by Lancashire and South Cumbria NHS Foundation Trust (LSCFT) who are referred to the L&D team. Participants will be recruited and allocated to intervention:control on a 1:1 basis. Interviews will be performed with 30–40 CYP in the intervention arm, 15 CYP in the control arm, up to 20 parents/guardians across both arms, up to 15 practitioners, and up to 10 site staff responsible for screening CYP for the trial. Intervention and control: Those allocated to the intervention will be offered SAU plus SFBT, and control participants will receive SAU only. Primary outcome: CYP frequency of offending behaviours assessed through the Self-Report Delinquency Measure (SRDM) at 12 months post-randomisation. Secondary outcomes: criminal offence data (national police database); emotional and behavioural difficulties (self-report and parent/guardian reported); gang affiliation (self-report). Process evaluation: evaluation of acceptability and experiences of the CYP, parents/guardians, site staff and practitioners; fidelity of SFBT delivery. Discussion This two-arm individually RCT will evaluate the effectiveness of SFBT in reducing offending behaviours in CYP presenting at police custody suites within the area served by LSCFT. Our process evaluation will assess the fidelity of delivery of SFBT, the factors affecting implementation, the acceptability of SFBT in CYP aged 10–17 years and recruitment and reach. We will also examine systems and structures for future delivery, therefore assessing overall scalability. Trial registration ClinicalTrials.gov ISRCTN14195235. Registered on June 16, 2023.
Do dogs preferentially encode the identity of the target object or the location of others' actions?
The ability to make sense of and predict others' actions is foundational for many socio-cognitive abilities. Dogs (Canis familiaris) constitute interesting comparative models for the study of action perception due to their marked sensitivity to human actions. We tested companion dogs (N = 21) in two screen-based eye-tracking experiments, adopting a task previously used with human infants and apes, to assess which aspects of an agent's action dogs consider relevant to the agent's underlying intentions. An agent was shown repeatedly acting upon the same one of two objects, positioned in the same location. We then presented the objects in swapped locations and the agent approached the objects centrally (Experiment 1) or the old object in the new location or the new object in the old location (Experiment 2). Dogs' anticipatory fixations and looking times did not reflect an expectation that agents should have continued approaching the same object nor the same location as witnessed during the brief familiarization phase; this contrasts with some findings with infants and apes, but aligns with findings in younger infants before they have sufficient motor experience with the observed action. However, dogs' pupil dilation and latency to make an anticipatory fixation suggested that, if anything, dogs expected the agents to keep approaching the same location rather than the same object, and their looking times showed sensitivity to the animacy of the agents. We conclude that dogs, lacking motor experience with the observed actions of grasping or kicking performed by a human or inanimate agent, might interpret such actions as directed toward a specific location rather than a specific object. Future research will need to further probe the suitability of anticipatory looking as measure of dogs' socio-cognitive abilities given differences between the visual systems of dogs and primates.
Adopting an anti-bullying programme for UK special schools
Bullying is a public health priority but to date, there is a lack of evidence-based anti-bullying programmes or interventions designed for use in special schools. KiVa is a successful anti-bullying programme for mainstream schools currently used in 23 countries. This brief paper outlines the co-development and adaptation of two KiVa lessons into KiVa-SEND lessons and their implementation in two special schools in the UK. One school supports pupils with a primary need of Autism, the other supports pupils with severe and complex learning disabilities. Engagement with the lessons was high from both pupils and staff; the content was perceived as acceptable by staff, complementing the curriculum and perceived as suitable for their pupils. Minor adjustments need to be made to ensure all pupils can comprehend and access the concepts. Further development of the KiVa-SEND programme and testing its potential effectiveness to reduce bullying and associated negative outcomes in special schools is now warranted.
Cost-effectiveness of therapist-assisted internet-delivered psychological therapies for PTSD differing in trauma focus in England: an economic evaluation based on the STOP-PTSD trial.
BACKGROUND: Although there are effective psychological treatments for post-traumatic stress disorder (PTSD), they remain inaccessible for many people. Digitally enabled therapy is a way to overcome this problem; however, there is little evidence on which forms of these therapies are most cost effective in PTSD. We aimed to assess the cost-effectiveness of the STOP-PTSD trial, which evaluated two therapist-assisted, internet-delivered cognitive behavioural therapies: cognitive therapy for PTSD (iCT-PTSD) and a programme focusing on stress management (iStress-PTSD). METHODS: In this health economic evaluation, we used data from the STOP-PTSD trial (n=217), a single-blind, randomised controlled trial, to compare iCT-PTSD and iStress-PTSD in terms of resource use and health outcomes. In the trial, participants (aged ≥18 years) who met DSM-5 criteria for PTSD were recruited from primary care therapy services in South East England. The interventions were delivered online with therapist support for the first 12 weeks, and three telephone calls over the next 3 months. Participants completed questionnaires on symptoms, wellbeing, quality of life, and resource use at baseline, 13 weeks, 26 weeks, and 39 weeks after randomisation. We used a cost-effectiveness analysis to assess cost per quality-adjusted life year (QALY) at 39 weeks post-randomisation, from the perspective of the English National Health Service (NHS) and personal social services and on the basis of intention-to-treat for complete cases. Treatment modules and the platform design were developed with extensive input from service users: service users also advised on the trial protocol and methods, including the health economic measures. This is a pre-planned analysis of the STOP-PTSD trial; the trial was registered prospectively on the ISRCTN Registry (ISRCTN16806208). FINDINGS: NHS costs were similar across treatment groups, but clinical outcomes were superior for iCT-PTSD compared with iStress-PTSD. The incremental cost-effectiveness ratio for NHS costs and personal social services was estimated as £1921 per QALY. iCT-PTSD had an estimated 91·6% chance of being cost effective at the £20 000 per QALY threshold. From the societal perspective, iCT-PTSD was cost saving compared with iStress-PTSD. INTERPRETATION: iCT-PTSD is a cost-effective form of therapist-assisted, internet-delivered psychological therapy relative to iStress-PTSD, and it could be considered for clinical implementation. FUNDING: Wellcome Trust and National Institute of Health Research Oxford Health Biomedical Research Centre.
VERBS OF PERCEPTION: A QUANTITATIVE TYPOLOGICAL STUDY
Previous studies have proposed that the lexicalization of perception verbs is constrained by a biologically grounded hierarchy of the senses. Other research traditions emphasize conceptual and communicative factors instead. Drawing on a balanced sample of perception verb lexicons in 100 languages, we found that vision tends to be lexicalized with a dedicated verb, but that nonvisual modalities do not conform to the predictions of the sense-modality hierarchy. We also found strong asymmetries in which sensory meanings colexify. Rather than a universal hierarchy of the senses, we suggest that two domain-general constraints—conceptual similarity and communicative need— interact to shape lexicalization patterns.
The effects of linear order in category learning: Some replications of Ramscar et al., (2010) and their implications for replicating training studies
Ramscar, Yarlett, Dye, Denny and Thorpe (2010) showed how, consis tent with the predictions of error-driven learning models, the order in which stimuli are presented in training can affect category learning. Specifically, learners exposed to artificial language input where objects preceded their labels learned the discriminating features of categories better than learners exposed to input where labels preceded objects. We sought to replicate this finding in two online experiments employing the same tests used originally: A four pictures test (match a label to one of four pictures) and a four labels test (match a picture to one of four labels). In our study, only findings from the four pictures test were consistent with the original result. Additionally, the effect sizes observed were smaller, and participants over-generalized high frequency category labels more than in the original study. We suggest that although Ramscar et al. [2010] feature-label order predictions were derived from error-driven learning, they failed to consider that this mechanism also predicts that perfomance in any training paradigm must inevitably be influ enced by participant prior experience. We consider our findings in light of these factors, and discuss implications for the generalizability and replication of training studies
Onomatopoeia, gestures, actions and words: How do caregivers use multimodal cues in their communication to children?
Most research on how children learn the mapping between words and world has assumed that language is arbitrary, and has investigated language learning in contexts in which objects referred to are present in the environment. Here, we report analyses of a semi-naturalistic corpus of caregivers talking to their 2-3 year-old. We focus on caregivers' use of non-arbitrary cues across different expressive channels: both iconic (onomatopoeia and representational gestures) and indexical (points and actions with objects). We ask if these cues are used differently when talking about objects known or unknown to the child, and when the referred objects are present or absent. We hypothesize that caregivers would use these cues more often with objects novel to the child. Moreover, they would use the iconic cues especially when objects are absent because iconic cues bring to the mind's eye properties of referents. We find that cue distribution differs: all cues except points are more common for unknown objects indicating their potential role in learning; onomatopoeia and representational gestures are more common for displaced contexts whereas indexical cues are more common when objects are present. Thus, caregivers provide multimodal non-arbitrary cues to support children's vocabulary learning and iconicity - specifically - can support linking mental representations for objects and labels.
Learning: Statistical Mechanisms in Language Acquisition
The grammatical structure of human languages is extremely complex, yet children master this complexity with apparent ease. One explanation is that we come to the task of acquisition equipped with knowledge about the possible grammatical structures of human languages—so-called “Universal Grammar”. An alternative is that grammatical patterns are abstracted from the input via a process of identifying reoccurring patterns and using that information to form grammatical generalizations. This statistical learning hypothesis receives support from computational research, which has revealed that even low level statistics based on adjacent word co-occurrences yield grammatically relevant information. Moreover, even as adults, our knowledge and usage of grammatical patterns is often graded and probabilistic, and in ways which directly reflect the statistical makeup of the language we experience. The current chapter explores such evidence and concludes that statistical learning mechanisms play a critical role in acquisition, whilst acknowledging holes in our current knowledge, particularly with respect to the learning of ‘higher level’ syntactic behaviours. Throughout, I emphasize that although a statistical approach is traditionally associated with a strongly empiricist position, specific accounts make specific claims about the nature of the learner, both in terms of learning mechanisms and the information that is primitive to the learning system. In particular, working models which construct grammatical generalizations often assume inbuilt semantic abstractions.
Poststroke Executive Function in Relation to White Matter Damage on Clinically Acquired CT Brain Imaging.
BACKGROUND: Executive function (EF) impairments are prevalent post stroke and are associated with white matter (WM) damage on MRI. However, less is known about the relationship between poststroke EF and WM damage on CT imaging. OBJECTIVE: To investigate the relationship between poststroke EF and WM damage associated with stroke lesions and WM hypointensities (WMHs) on clinically acquired CT imaging. METHOD: This study analyzed data from the Oxford Cognitive Screening Program, which recruited individuals aged ≥18 years with a confirmed stroke from an acute stroke unit. The individuals completed a follow-up assessment 6 months post stroke. We included individuals with a CT scan showing a visible stroke who completed follow-up EF assessment using the Oxford Cognitive Screen-Plus rule-finding task. We manually delineated stroke lesions and quantified then dichotomized WM damage caused by the stroke using the HCP-842 atlas. We visually rated then dichotomized WMHs using the Age-Related White Matter Changes Scale. RESULTS: Among 87 stroke survivors (M age = 73.60 ± 11.75; 41 female; 61 ischemic stroke), multivariable linear regression showed that stroke damage to the medial lemniscus ( B = -8.86, P < 0.001) and the presence of WMHs ( B = -5.42, P = 0.005) were associated with poorer EF 6 months post stroke after adjusting for covariates including age and education. CONCLUSION: Poorer EF was associated with WM damage caused by stroke lesions and WMHs on CT. These results confirm the importance of WM integrity for EF post stroke and demonstrate the prognostic utility of CT-derived imaging markers for poststroke cognitive outcomes.
Do we really need a new definition of dyslexia? A commentary.
We provide a commentary on current debates about the definition of dyslexia. We agree with others that dyslexia is best thought of as a dimensional disorder with the best established causal risk factor being a deficit in phonological processing. Dyslexia is particularly common in children from families with a history of dyslexia and in children with preschool language difficulties. We argue that definitions may differ depending upon their purpose. Traditional discrepancy definitions may be useful for research purposes, but when considering the provision of educational services discrepancy definitions are not useful since all children with reading difficulties require reading intervention regardless of their level of IQ.