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We investigated the role of the septo-hippocampal cholinergic projection in anxiety, spatial novelty preference, and differential reward for low rates of responding (DRL) performance. Cholinergic neurons of the rat medial septum (MS) and the vertical limb of the diagonal band of Broca (VDB) were lesioned using the selective immunotoxin, 192 IgG-saporin. Rats were then tested on several behavioral tests previously shown to be sensitive to either (a) hippocampal lesions or (b) nonselective MS/VDB lesions which target both cholinergic and γ-aminobutyric acid (GABA)-ergic projections, or both. Saporin lesions substantially reduced hippocampal cholinergic innervation, resulting in an absence of acetyl cholinesterase staining and markedly reduced choline acetyltransferase activity (mean reduction: 80 ± 5%; range: 50-97%). However, the saporin-lesioned rats did not differ from control rats in any of the behavioral tests. Thus we found no evidence from these lesion studies that the septo-hippocampal cholinergic projection plays an essential role in anxiety, spatial novelty preference, or DRL.

Original publication

DOI

10.1037/bne0000072

Type

Journal article

Journal

Behav Neurosci

Publication Date

08/2015

Volume

129

Pages

491 - 501

Keywords

Acetylcholine, Acetylcholinesterase, Animals, Anxiety, Choline O-Acetyltransferase, Cholinergic Neurons, Diagonal Band of Broca, Exploratory Behavior, Hippocampus, Male, Motor Activity, Neural Pathways, Protein Synthesis Inhibitors, Rats, Reward, Ribosome Inactivating Proteins, Type 1, Septal Nuclei